Product Name:
Rat Sphingomyelin Phosphodiesterase 2 (NSMASE) ELISA Kit
Cat.#:
JL17826
Regulatory Status:
Alternative:
SMPD2; N-SMase; ISC1; Neutral Sphingomyelin Phosphodiesterase; Sphingomyelin Phosphodiesterase 2;Neutral Membrane; Lyso-platelet-activating factor-phospholipase C;Neutral Sphingomyelinase
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.08ng/mL
Specificity:
This kit recognizes Rat NSMASE in samples. No significant cross-reactivity or interference between Rat NSMASE and analogues was observed.
Sample Type:
tissue homogenates;cell lysates and other biological fluids
Assay Procedure:
Research Area:
Cell Biology
Size & Price: 
Product Datasheets
Instruction: 
| NSMASE
Sphingomyelin phosphodiesterase 2 is an enzyme that is encoded by the SMPD2 gene.This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase.
| Repeatability
Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%| Recovery
The recovery of Rat NSMASE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Tissue Homogenates(n=8) | 85-102 | 96 |
| Cell Lysates(n=8) | 92-105 | 101 |
| Linearity
Samples were spiked with high concentrations of Rat NSMASE and diluted with Universal Diluent to produce samples with values within the range of the assay.| Tissue Homogenates(n=4) | Cell Lysates(n=4) | ||
| 1 : 2 | Range (%) | 85-95 | 88-96 |
| Average (%) | 93 | 94 | |
| 1 : 4 | Range (%) | 89-103 | 87-108 |
| Average (%) | 98 | 100 |
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